FIGURE 5.

Differentiation of RD cells via transfection of a miRNA miR-206 mimetic increases the frequency of chromosome 2 positioning along the major nuclear axis. (A) Immunohistochemistry for myosin heavy chain, a marker of myogenesis, shows an increase in its expression and the formation of elongated cellular shapes, some of which exhibit multi-nucleation, both consistent with differentiation of RMS cells specifically in those cells transfected with the miR-206 mimetic and not the negative control mimetic. (B) Representative images of chromosome 2 visualization via hybridization of chromosome paint in RD cells. (C) The proportion of chromosome 2 assigned as being associated either with the minor axis (minor parallel) or the major axis (major parallel) in RD cells transfected as indicated. The number within the bars indicates the value assigned to the minor parallel for each condition. (D) The proportion of cells with chromosome 2 positioning as indicated on a per-cell basis. The percentage of cells with <50% of chromosome signals along the major axis is indicated within the bars. For both 5C and 5D, statistical testing was performed using Fisher’s exact test for proportions. **: p < 0.01. n = 76 and 61 nuclei for negative control and miR-206 nuclei, respectively.