FIG. 6.

Southern hybridization of total DNA of selected toluene and benzene degraders from wells S1-11 and S1-180 and of JS705 with probes for the clc-mcbF genes (insert of pCBA24) (A), for the mcbAB genes (pCBA31) (B), and for 16S rDNA (C). All DNAs were digested with PstI. Lane 1, strain JS743; lane 2, strain JS751; lane 3, strain JS745; lane 4, strain JS744; lane 5, strain JS749; lane 6, strain JS705. The positions of size markers (1-kb ladder) are indicated on the left. An interpretation of the hybridization results is shown at the bottom.