TABLE 1.

Sampling dates and summary of studies performed at KAFB

Well	Results for the following sampling datesa:
	August 1992	January 1993	August 1994	September 1996	December 1996	January 1998
S1-1	I+, JS705 isolated	I+	E+, CH+, SH+, PCR+	—b	I−	I+
S1-2	I−	I−	E−, CH+/−, SH−, PCR+/−		I+, PCR+	I−, E−
S1-10				I+, CH+, SH+
S1-11				I+, CH+, SH+
S1-16					I−, E−
S1-18					I−, E−
S1-179					I−, E−
S1-180				I−, SH−, PCR+		I−, E−

^aI+, CB, benzene, and toluene degraders were isolated; I−, benzene and toluene degraders were isolated but CB degraders were not isolated; E+, positive for enrichment on CB, benzene, toluene, and 1,4-dichlorobenzene but not for enrichment on 3-CBA; E−, no enrichment on CB, 1,4-dichlorobenzene, or 3-CBA but positive for toluene or benzene; CH+, colony hybridization occurred with clc and mcb probes of JS705; CH+/−, colony hybridization occurred with either the clc probe or the mcb probe; SH+, hybridization to clc, mcb, or 16S rDNA probes of isolated strains in Southern analysis identical to hybridization of strain JS705; SH−, no hybridization or different hybridization patterns; PCR+, PCR-amplified fragment from total DNA isolated from well water positive with all primers specific for JS705; PCR+/−, fragment positive with only one primer set. 

^b—, well was dry.