TABLE 3.
Primers used
| Primer set | Primer | Sequence (5′-3′) | Product size | Locationa |
|---|---|---|---|---|
| clc | CGAGTTGCCGAGGTCGCAGGC | 763 bp | +9-29 | |
| TCATGCCACTGTCTCCGTAGCCG | −772-750 | |||
| mcb | GTCAGGAAGGCGGGCGACTACTTTAC | 617 bp | +183-209 | |
| GATATAGAAGCCGCTGCCATGTC | −800-777 | |||
| clcA-mcbF | GGGCAAATGGGTGGACGATGACTG | 839 bp | +626-649 | |
| GCGGCGCCGGGACAACTC | −170-153 | |||
| 16S rDNA | V1.1 | GCGGCGTGCCTAATACATGC | 659 bp | +41-60 |
| V3.2 | ATCTACGCATTTCACCGCTAC | −705-685 | ||
| 16S rDNA | 6F | GGAGAGTTAGATCTTGGCTCAG | 1.5 kb | +6-28 |
| 1510R | GTGCTGCAGGGTTACCTTGTTACGACT | −1510-1483 |
a
Location relative to the start site of the gene.