Fig. 1.

BDMC administration alleviates LPS-induced ALI in mice. Mice were intragastrically administered with BDMC (100 mg/kg/day) for 3 days, and then intratracheally injected with a single dose of LPS (5 mg/kg). Mice were sacrificed 12 h post-LPS injection with the lungs collected for further examination. (A) H&E staining and lung inflammation score were used to detect the lung histopathological changes (n = 6). (B) TUNEL staining and apoptotic index were used to evaluate cell apoptosis (n = 6). (C) The LDH activity in lungs (n = 6). (D) The lung wet to dry ratio (n = 6). (E) The total proteins in BALF (n = 6). (F-G) The pulmonary function as determined by airway resistance and pulmonary ventilation (n = 6). (H-J) The arterial blood gas analysis of PaO₂, PaCO₂ and HCO₃⁻ (n = 6). Values represent the mean ± SD. *P<0.05 versus Saline + Vehicle; ^#P<0.05 versus LPS + Vehicle