Fig. 3.

BDMC administration alleviates oxidative stress in ALI mice. Mice were intragastrically administered with BDMC (100 mg/kg/day) for 3 days, and then intratracheally injected with a single dose of LPS (5 mg/kg). Mice were sacrificed 12 h post-LPS injection with the lungs collected for further examination. (A) The ROS generation in lung tissues were assessed by a DCFH-DA probe (n = 6). (B-C) The levels MDA and GSH in murine lungs (n = 6). (D) The western blot images and relative statistical results of NRF2 and SOD2 (n = 6). (E) The levels of total SOD activity and NRF2 activity (n = 6). Values represent the mean ± SD. *P<0.05 versus Saline + Vehicle; ^#P<0.05 versus LPS + Vehicle