Fig. 2.

Ovariectomy leads to systemic dyslipidemia and cognitive impairment in LDLR ^−/− mice. C57BL/6 and LDLR^−/− mice were fed a normal diet (ND) or a high-fat diet (HFD) for 90 days. Bilateral ovariectomy was performed in LDLR^−/− mice fed a HFD to simulate a postmenopausal stage. A Serum of mice was obtained after 90 days of administration, and the levels of TC, TG, LDL-c and HDL-c were detected using biochemical kits. B Livers of mice were obtained after 90 days of administration, and the levels of TC and TG in liver homogenates were detected using biochemical kits. C The hippocampi of mice were obtained after 90 days of administration, and the levels of TC and TG in hippocampal homogenates were detected using biochemical kits. D The learning and memory ability of mice was evaluated by the novel object recognition test after 4, 8, and 90 days of administration. E Spatial memory of mice was evaluated by the Y-maze task after 4, 8, and 90 days of administration. F–H The learning and memory abilities of mice were evaluated by the Morris water maze after 4, 8, and 90 days of administration. F indicates the escape latency of mice on the fifth training day of the Morris water maze test. G Indicates the number of platform crossings on the sixth day of the Morris water maze test. H Shows the swimming paths of mice on the first and fifth training days of the Morris water maze test. N = 5. In the indicated comparison, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001