Fig. 6.

ERα, ERβ, and GPER in common mediate the protective effect of lipid-lowering agents on SH-SY5Y cells. A In PA-treated SH-SY-5Y cells, AB23A was used alone or in combination with G-15, MPP or PHTPP for 24 h, and cell viability was detected by MTT assay. B G-15, MPP, or PHTPP were paired and acted together with AB23A on PA-treated SH-SY-5Y cells for 24 h. The expression levels of Bax and Bcl-2 were detected by western blot. C Related to B, the relative quantitative analysis of Bax and Bcl-2 expression in SH-SY-5Y cells. D G-15, MPP, or PHTPP were paired and acted together with AB23A on PA-treated SH-SY-5Y cells for 24 h. The expression levels of PSD-95 and Tau were detected by immunofluorescence. E Related to D, the quantitative analysis of the mean optical density of PSD-95 and Tau expression in SH-SY-5Y cells. F SH-SY-5Y cells were treated with G-15, MPP and PHTPP combined with AB23A and treated with PA for 24 h. The expression levels of Bax, Bcl-2, PSD-95 and Tau were detected by western blotting. G Relative quantitative analysis of Bax, Bcl-2, PSD-95 and Tau expression in SH-SY-5Y cells. H SH-SY-5Y cells were treated with G-15, MPP and PHTPP combined with AB23A and treated with PA for 24 h. The expression levels of PSD-95 and Tau were detected by immunofluorescence. I Related to H, the quantitative analysis of the mean optical density of PSD-95 and Tau expression in SH-SY-5Y cells. J SH-SY-5Y cells incubated with G-15, MPP and PHTPP in combination with AB23A and treated with PA for 24 h. Cell viability was detected by MTT assay. A and J, n = 6. B–I, n = 3. In the indicated comparison, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001