Table 2.

Primer and Probe Sets for RB1 and GJB2 Target Regions

	Forward primer	Reverse primer	Methylated probe	Unmethylated probe
RB1	5′-GTTTAAGGAG GGAGAGTGG-3′	5′-AAATAACTATAAA CCTCATCCCTATCC-3′	FAM5′-AACACGTCCGAACCG CGCCGAATAC3′-TAMRA	VIC5′-AACACATCCAAACC ACACCAAATAC3′-TAMRA
GJB2	5′-GGAATTGATTT ATTTTTTGGAG-3′	5′-AAAAAAACCACTA AAATCTTAACCC-3′	FAM-5′CCGAAATCGACT AACTCCGCGTTA3′-TAMRA	VIC-5′CCAAAATCAACTAA CTCCACATTA3′-TAMRA