Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 11;101(45):e31725. doi: 10.1097/MD.0000000000031725

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc.

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

The regulation of PVT1 on chondrocytes depends on the regulation of the miR-497/AKT3 axis. (A) MTT assay indicated that transfection of either miR-497-inhibitor or sh-AKT3 reversed the effects of si-PVT1 transfection on the proliferation of IL-1β-induced chondrocytes. (B) FCM revealed that transfection of either miR-497 inhibitor or sh-AKT3 reversed the effects of si-PVT1 transfection on apoptotic rates of IL-1β-induced chondrocytes. (C) WB analysis revealed that transfection of either miR-497-inhibitor or sh-AKT3 reversed the effects of transfection of si-PVT1 on aggrecan, collagen, and MMP-9 expressions in IL-1β-induced chondrocytes. Data are expressed as mean ± standard deviation (mean ± SD); n = 24; *P < .05 versus IL-1β + si-NC group; ^#P < .05 versus IL-1β + si-PVT1 + miR-497 inhibitor group; ^$P < .05 versus IL-1β + si-PVT1 + sh-AKT3 group. IL-1β = Interleukin-1 beta, PVT1 = plasmacytoma variant translocation 1.