Detection of STEC in prespiked ground beef by the AmpliSensor PCR assay: extent of amplification versus cycle number and initial copy number of target DNA. Symbols: ■, no STEC; +, 3 CFU/25 g; •, 6 CFU/25 g; □, 33 CFU/25 g; ×, 66 CFU/25 g; ▴, 336 CFU/25 g.