TABLE 4.
Detection of STEC in naturally contaminated raw milk samples by the AmpliSensor PCR assay and the culture method
| Sample | Detection indexa | STEC isolated |
|---|---|---|
| VE2-58 | 0.59 | Yes |
| VE4-11 | 0.68 | Yes |
| VE4-31 | 0.71 | Yes |
| VE5-7 | 0.72 | Yes |
| VE5-18 | 0.66 | Yes |
| VE7-34 | 0.64 | Yes |
| VE7-46 | 0.55 | No |
| VE11-38 | 0.73 | Yes |
| VE12-59 | 0.77 | Yes |
| VE14-67 | 0.68 | Yes |
| VE15-56 | 0.53 | Yes |
| VE16-37 | 0.69 | Yes |
| VE16-64 | 0.64 | Yes |
| VE16-75 | 0.73 | Yes |
| VE20-28 | 0.66 | Yes |
| VE21-45 | 0.68 | No |
| VE22-120 | 0.7 | Yes |
| STEC strain 933W | 0.7 | Yes |
| E. coli ATCC 25922 | 0 | No |
a
Detection index values were determined at the end of 25 cycles of seminested amplification. All remaining 31 samples had detection index values within 0.0 ± 0.10, and no STEC were isolated by the culture method.