Author response table 1. Comparing with the sequences used in references 26 and 28, we have the following timing parameters.

	Sequence	Frequency (Hz)	N Compound	Acquisition time (s)	Pause (s)	Frame rate (Hz)
Macé et al.,	High sensibility	500	200	0.4	0.2	1.7
Neuron, 2018	High speed	500	50	0.1	0.1	10
Brunner et al.,	High sensibility	500	250	0.5	0	2
Nat Prot, 2021	High speed	500	50	0.1	0	10
This study		500	250	0.5	0.3	1.25