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. 2023 Sep 4;193(4):2337–2360. doi: 10.1093/plphys/kiad484

Figure 4.

Figure 4.

Distribution of ROS in root hairs of Col-0 and bulges of rhd2-1 and der1-3 mutants after staining with Amplex Red. A–I) Growing root hair of Col-0 (A–C), developing bulge of rhd2-1 mutant (D–F), and der1-3 mutant (G–I). Root hairs and bulges were imaged at time points of 0 min (A, D, G), 5 min (B, E, H), and 10 min (C, F, I) of growth. Fluorescence intensity distribution is visualized in a pseudocolor-coded scale, where black represents minimal intensity and white represents maximum intensity (insets in A, D, G). J) Mean fluorescence intensity distribution of Amplex Red measured along a 10-µm line oriented longitudinally at the central part of Col-0 growing root hairs, reaching the apical cell wall (schematically illustrated in Supplemental Fig. S1A). Error bars represent standard deviation (SD). K) Semiquantitative mean Amplex Red fluorescence intensity analysis (measured area is schematically illustrated in Supplemental Fig. S1C) in bulges of rhd2-1 and der1-3 mutants, and respective Col-0 and C24 wild types. N = 10 (J, K). Box plots display the first and third quartiles, split by the median; the crosses indicate the mean values; whiskers extend to include the max/min values. Lowercase letters indicate statistical significance between lines according to one-way ANOVA with Fisher's LSD tests (P < 0.05). Scale bar = 10 µm (A to I).