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. 2023 Sep 4;193(4):2337–2360. doi: 10.1093/plphys/kiad484

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© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Single particle dynamic colocalization analysis of GFP-labeled mitochondria with ROS-accumulating compartments after staining with CellROX Deep Red in root hairs. A to C) Three different time points from time-lapsed video-recordings of growing root hairs containing GFP-tagged mitochondria (in green) and ROS-accumulating compartments (in magenta) after CellROX Deep Red staining. D) Video-sequence captured from dashed box in (A) showing mitochondria (green arrows) and ROS-accumulating compartments (magenta arrows) colocalized (double arrows in merge), while small individual mitochondria in the clear zone are still free of ROS (green arrows only in merge). E) Video-sequence captured from dashed box in (B) showing small mitochondrion negative for ROS staining (green arrow) independent from larger mitochondria containing ROS (double arrows in merge). F) Video-sequence captured from dashed box in (C) showing large round and immobile mitochondria containing ROS (double arrows in merge) and small mobile individual mitochondria free of ROS (green arrows only in merge). Time sequences (in seconds) are shown in mito-GFP channels (D to F). Scale bar = 5 µm (A to C), 1 µm (D to F).