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. 2023 Sep 4;193(4):2337–2360. doi: 10.1093/plphys/kiad484

Figure 8.

Figure 8.

Colocalization analysis of subcellular molecular markers in root hairs with compartments accumulating ROS after staining with Amplex Red. A–C) Colocalization with transgenic line for visualization of vesicular compartments containing GFP-RHD2, a GFP-tagged AtRBOHC/RHD2. D–F) Colocalization with transgenic line carrying early endosomal marker GFP-RabA1d. G–I) Colocalization with transgenic line carrying late endosomal marker RabF2a-YFP. J–L) Colocalization with transgenic line carrying GFP-tagged mitochondria. Overview of root hairs carrying molecular markers (in green) and ROS-accumulating compartments (in magenta) after Amplex Red staining (A, D, G, J). Detailed images of compartments containing molecular markers and ROS (B, E, H, K) from the white boxes shown in (A, D, G, J). Fluorescence intensity profiles (C, F, I, L) of fluorescence signals of molecular markers (green lines) and ROS-containing compartments (magenta lines) along the interrupted white lines shown in (B, E, H, K). Arrows in (B, C, E, F, H, I, K, L) indicate the position of the compartments. RabF2a-YFP is artificially displayed in green. Scale bar = 5 µm (A, D, G, J), 0.5 µm (B, E, H, K).