Fig. 3.

Regulation of LOXL2 expression by NF-kB activation through glucose metabolism in gemcitabine-resistant pancreatic cancer cells. A The mRNA levels of the GLUT genes were quantified by qPCR in Mia con and Mia GR. B Comparison of 2-NBDG uptake between Mia Con and Mia GR (no treatment, WZB117 treatment, siGLUT3 transfection) by flow cytometry histograms and graphs. C Comparison of lactate production between Mia Con and Mia GR (no treatment, WZB117 or 2DG treatment, siGLUT3 transfection (D)). E The mRNA levels of GLUT3 and LOXL2 in Mia GR and Mia GR transfected with siGLUT3. F The mRNA levels of LOXL2 in Mia Con and Mia GR (no treatment, WZB117 or 2DG treatment). G and K Mia GR cells were transfected with the control or GLUT3 siRNA. GLUT3, LOXL2 G, and p-NF-κB, NF-κB, and GLUT3 K were analyzed by immunoblotting. H and J 2-DG and WZB117 were treated with Mia GR cells and cell lysates were immunoblotted. I The phospho NF-κB and NF-κB protein expression in Mia Con and Mia GR were processed by immunoblotting