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. 1983 Jul;72(3):821–824. doi: 10.1104/pp.72.3.821

Lysine Transport in Two Barley Mutants with Altered Uptake of Basic Amino Acids in the Root 1

Simon W J Bright 1,2, Joseph S H Kueh 1,2, Sven E Rognes 1,2
PMCID: PMC1066327  PMID: 16663092

Abstract

Amino acid uptake was examined in two barley (Hordeum vulgare L.) mutants R906 and R4402 which had been selected as resistant to the lysine analog S-(2-aminoethyl)-cysteine. The mutants were found to be allelic by crossing and examination of F1 and F2 progeny. The mutant genes were designated aec1a and aec1b, respectively. The uptake of the basic amino acids lysine, arginine, and ornithine from 50 micromolar solutions was strongly decreased in roots of the mutants, whereas uptake of neutral and acidic amino acids was unaffected. The pattern of uptake of lysine over the range 10−7 to 10−2 molar was consistent with there being, principally, two uptake systems operating for basic amino acids in roots and that a low-concentration, high-affinity system is reduced or lacking in the mutants. The residual transport activity in the mutants had a different relative affinity for lysine and arginine to the wild-type system. Uptake of lysine by leaf slices was unimpaired in the mutants suggesting that the leaf uptake system is unaffected by the aec1 gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fricke U. Tritosol: a new scintillation cocktail based on Triton X-100. Anal Biochem. 1975 Feb;63(2):555–558. doi: 10.1016/0003-2697(75)90379-6. [DOI] [PubMed] [Google Scholar]
  2. Harrington H. M., Henke R. R. Amino Acid Transport into Cultured Tobacco Cells: I. LYSINE TRANSPORT. Plant Physiol. 1981 Feb;67(2):373–378. doi: 10.1104/pp.67.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kinraide T. B., Etherton B. Electrical evidence for different mechanisms of uptake for basic, neutral, and acidic amino acids in oat coleoptiles. Plant Physiol. 1980 Jun;65(6):1085–1089. doi: 10.1104/pp.65.6.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kinraide T. B. Interamino Acid Inhibition of Transport in Higher Plants : EVIDENCE FOR TWO TRANSPORT CHANNELS WITH ASCERTAINABLE AFFINITIES FOR AMINO ACIDS. Plant Physiol. 1981 Dec;68(6):1327–1333. doi: 10.1104/pp.68.6.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Maretzki A., Thom M. Arginine and lysine transport in sugarcane cell suspension cultures. Biochemistry. 1970 Jun 23;9(13):2731–2736. doi: 10.1021/bi00815a022. [DOI] [PubMed] [Google Scholar]
  6. Niven D. F., Jeacocke R. E., Hamilton W. A. The membrane potential as the driving force for the accumulation of lysine by Staphylococcus aureus. FEBS Lett. 1973 Feb 1;29(3):248–252. doi: 10.1016/0014-5793(73)80030-4. [DOI] [PubMed] [Google Scholar]

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