FIG. 4.
Autoradiograms of two-dimensional gels obtained from cultures of E. faecalis labeled with 35S during incubation in tap water. Growth, labeling, and gel running were performed in two independent experiments and led to comparable results. (A) Reference gel obtained from a culture labeled during the exponential-growth phase. (B to D) Gels from cultures incubated for 1 day, 3 days, and 2 weeks in the microcosm, respectively. Arrows indicate the proteins which are relatively induced over the level of growing cultures or specific for the starvation stress. The intense spot to the right of O29 indicates a polypeptide with a spot intensity that is comparable in growing and starved cultures and is independent of the length of incubation in the oligotrophic environment. See the text for a more detailed explanation.