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. 2023 Nov 8;17:1308972. doi: 10.3389/fncel.2023.1308972

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Copyright © 2023 Morisaki, Ohshima, Suzuki and Misawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lymphocyte activation gene-3 (LAG-3) knockdown promotes inflammatory activation by IFN-γ. BV2 cells were transfected with scramble control or LAG-3 siRNA. After incubation with the siRNAs, IFN-γ was added and the cells were harvested. (A) LAG-3 mRNA level was measured using quantitative real-time PCR to confirm the efficiency of knockdown by siRNA. (B) iNOS mRNA was measured in the siRNA-treated BV2 cells. (C) NO concentration was determined in culture supernatants of the siRNA-treated BV2 cells. Statistical analyses were performed using two-way ANOVA, followed by Tukey–Kramer post-hoc test; **p < 0.01, ****p < 0.0001. Error bars represent SEM.