Extended Data Fig. 8 ∣. Investigation of glycoRNA levels during HL-60 differentiation.
(a) CLSM images of U1, U3, and U8 glycoRNA levels evaluated by ARPLA in HL-60 and dHL-60 cells; (b) CLSM images of HL-60, dHL-60 cells in control groups using DNA with scrambled sequences; (c) Quantitative analysis for relative fluorescence intensity of ARPLA in (a) and (b). Data in (c) are representative of three independent experiments, n = 5 frames. Data are mean ±S.D. The statistical significance is determined by unpaired two-tailed Student’s t-test as (ns) not significant, (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001. P (U1 HL60 vs. dHL60) = 0.0159, P (U3 HL60 vs. dHL60) = 0.0079, P (U8 HL60 vs. dHL60) = 0.0002.