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. Author manuscript; available in PMC: 2024 Apr 17.
Published in final edited form as: Nat Biotechnol. 2023 May 22;42(4):608–616. doi: 10.1038/s41587-023-01801-z

Extended Data Fig. 9 ∣. Fluorescence imaging of bulk sialic acid on the cell surface of THP-1 monocyte, resting M0 macrophage (M0), LPS activated M0 macrophage (M0 + LPS).

Extended Data Fig. 9 ∣

(a) Representative images of total sialic acid. These cells were metabolically labeled by Ac4ManNAz for 24 h, followed by incubation with DBCO-PEG4-biotin and Cy5-streptavidin for fluorescence imaging. Scale bars for cell image: 50 μm. (b) Quantification of the mean fluorescent intensity of the images, n = 3 biological replicates. Data are mean ±S.D. Unpaired two-tailed Student’s t-test determines the statistical significance. (**) p = 0.0013, (*) p = 0.0469.