Skip to main content
. Author manuscript; available in PMC: 2024 Apr 17.
Published in final edited form as: Nat Biotechnol. 2023 May 22;42(4):608–616. doi: 10.1038/s41587-023-01801-z

Fig. 3 ∣. Spatial distributions of glycoRNAs revealed by ARPLA in HL-60 cells.

Fig. 3 ∣

a, Representative fluorescence imaging of lipid rafts, indicated by Alexa Fluor 555-labeled CT-B (top) or BODIPY dye-labeled sphingolipids (bottom), and glycoRNAs, imaged by ARPLA with U1 probe. The overlay images and plot profiles display the colocalization between glycoRNAs and lipid rafts; Pearson’s coefficients: r = 0.572 ± 0.130 (CT-B) and r = 0.539 ± 0.163 (BODIPY); scale bar, 5 μm. The intensities of different fluorochromes along the yellow lane in the overlay images are shown in the histograms on the right. b, Representative immunofluorescence images of t-SNARE (top) and v-SNARE (bottom) with glycoRNAs, indicating the intracellular trafficking of glycoRNAs. The overlay images and plot profiles show colocalization between glycoRNAs and t-SNARE (Pearson’s coefficient; r = 0.70) or v-SNARE (Pearson’s coefficient: r = 0.58); scale bar, 5 μm. The intensities of different fluorochromes along the yellow line in the merged images are shown in the histograms on the right. All experiments were repeated three times independently with similar results.