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. 2023 Nov 21;13:20397. doi: 10.1038/s41598-023-47582-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

RPA Basic Primer Screening and Optimization. Target HPV gBlock DNA and no-target human genomic DNA were first amplified in RPA, and the resultant amplicons were diluted and analyzed in qPCR using the same primer pair as for the RPA reaction. The relative amplification yield of positive reactions, containing 50 or 100 input copies of target HPV DNA, to no-target reactions is displayed for primer pairs specific to (a) HPV16, (b) HPV18 and (c) HPV45. Data represent the mean relative amplification yield for nine replicates (three RPA replicates x three technical replicates). Outlined boxes indicate primer pairs with the greatest relative amplification yield for both 50 and 100 input copies of target HPV DNA that were chosen for tailed primer design.