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. 2023 Nov 21;13:20397. doi: 10.1038/s41598-023-47582-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RPA tailed primer screens. Lateral flow strips (left) of RPA products from tailed primers designed for (a) HPV16, (b) HPV18, and (c) HPV45. Signal-to-background ratio (SBR) from each strip (right) and mean relative amplification yield (n = 9; three RPA replicates x three technical replicates) are displayed above each primer pair. Starred replicates indicate nonspecific products, confirmed by melt curve analysis, which produced a positive visual signal. Tailed primer pairs chosen for further assay development are highlighted. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns = not significant; significance determined for each primer pair by a two-tailed unpaired t-test.