Figure 4.

NATflow overview. (a) NATflow instrument and associated consumables needed to run the tailed primer HPV assay. (b) Schematic of NATflow-compatible lateral flow strip. Lateral flow strips for integrated testing on the NATflow were modified from¹⁹ to decrease running time, reduce strip-to-strip variability, and increase manufacturability at scale. (c) Custom PCR tube insert for multiplexed testing on the NATflow. The insert fits inside a high-profile PCR tube and divides the reaction space into three wells that each hold up to 15 µL. The insert allows reactions for HPV16, HPV18, and HPV45 to be spatially separated in a single NATflow cartridge, thus reducing cost and complexity associated with running multiple reactions simultaneously.