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. 2023 Nov 21;13:20397. doi: 10.1038/s41598-023-47582-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Integrated cartridge performance with custom PCR insert. (a) Reactions containing gDNA or extracted cellular DNA from SiHa (HPV16-positive), HeLa (HPV18-positive), and/or MS751 (HPV45-positive) cells at the indicated input concentrations were run in triplicate in the NATflow cartridges with the custom PCR insert. Highlighted images indicate invalid tests due to cartridge failures that were excluded from analysis. (b) Mean ± standard deviation of the signal generation at test lines, as determined by the AX-2X-S lateral flow reader of the replicates shown in (a). Circles represent individual replicates, and circles beneath the x-axis indicate expected 16 or 18/45 positivity. Signal above the positivity threshold (dashed line) is only seen at test lines when the target HPV genotype is present.