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. 2023 Nov 21;14:7353. doi: 10.1038/s41467-023-42994-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Total lipid fraction extracted from HeLa cells transfected with vectors or plasmids encoding the indicated Gag and Gag derivatives was separated in a solvent for polar lipids (a) or neutral lipids (c) as described in “Methods”. The bands corresponding to SM (a) and Chol (c) indicated by arrowheads on the right side of thin layer chromatography (TLC) plates were quantitated. The relative band intensity to the control (vectors) was shown as a mean ± SD for three independent experiments in (b) and (d). The values in each experiment were also shown in dots in three different blue. The differences were not significant (ns) among all samples at the significance level of 0.05 in one-way ANOVA post-hoc Tukey test. Band positions of phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylethanolamine (PE), and cholesteryl ester (CE) were indicated by arrowheads on the left side of TLC plates in (a) and (c). Vec, vectors; WT, Gag WT; ∆L, Gag-∆L; P99A, Gag-P99A; EE, Gag-EE; WM, Gag-WM. Source data and statistics for (b and d) were provided as a Source Data file.