Fig. 1. Task and behavior.
a, b Mice are trained on a modified trace eyeblink conditioning task (left) where the inter-stimulus time interval (ISI) between a flash of light and a transient periocular airpuff on a paired trial is sampled from a ‘Narrow distribution’ with a single ISI (350 ms, blue, top) or sampled from a discrete uniform ‘Wide distribution’ (200, 275, 350, 425, 500 ms, Green, middle). The airpuff is omitted in test trials (right, bottom panel) to observe the predictive component of the eyeblink response. c Eyelid closure is measured on test and paired trials to determine the amplitude (ACR) of the predictive response (blue) and distinguish it from the reflexive response (gray) at the time of the airpuff. d Normalized single-trial eyelid closure traces for paired (above, blue/green lines) and test trials (below, gray lines) for the Narrow and Wide conditions. The traces are aligned to the onset time of the light cue (black dashed line) and the time of the airpuff(s) is also indicated (black dashed lines). e Examples of average normalized eyelid traces for test trials in the Narrow (blue) and Wide (green) conditions. Note that no airpuff was administered in these trials but the mean of both interval conditions is indicated (black dashed line at 350 ms). Error bars indicate standard error.