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. 2023 Oct 24;31:101142. doi: 10.1016/j.omtm.2023.101142

Figure 2.

Figure 2

Comprehensive physicochemical characterization of high- and low-density particles

(A) CsCl density-gradient-analytical ultracentrifugation (CsCl-DG-AUC) profile of Bulk, F1.1 and F2.2 fractions. (B) Capillary gel electrophoresis (CGE) electropherograms of encapsidated DNA in F1.1 and F2.2 particles. (C) CGE electropherogram of F1.1 and F2.2 particles for capsid viral protein (VP) components. (D) Magnified image of electropherogram shown in (C). (E) VP stoichiometry of Bulk, F1.1, and F2.2 fractions. To determine the VP molar stoichiometry, areas of the CGE peaks detected at 214 nm were divided by the molar extinction efficient of VPs at 214 nm, reflecting the UV absorbance of peptide bonds and amino acids under denatured conditions.