Figure 2.

Characterization of hematopoietic lineage cells derived from iPSCs

(A) Representative photographs showing the morphological changes observed during the differentiation of iPSCs toward iHSPCs and myeloid lineage cells. One photo for each time point is shown (scale bar: 100 μm).

(B) Frequency of CD34⁺ and CD45⁺ cells (upper row) and cumulative CD34⁺ and CD45⁺ cell yield (lower row) was assessed at different time points during iPSCs differentiation toward hematopoietic lineage cells.

(C) Proliferation ability and differentiation potential of iHSPCs were assessed in vitro by Colony-forming units (CFUs) assay. The number of Burst-forming unit-erythroid (BFU-E), granulocyte-macrophage progenitor cells (CFU-GM), and multipotential granulocyte, erythroid, macrophage and megakaryocyte progenitor cells (CFU-GEMM, or CFU-Mix) was evaluated at different time points during iPSC differentiation. Data are expressed as number of colonies (x10⁴)/mL. Data are presented as mean ± SD (n = 6). Statistical significance was calculated using ANOVA. See also Figures S1 and S2.