Validation of iPSC-derived monocytes (iMono)
(A) Total number of iHSPCs and iMono generated during the differentiation of iPSCs of healthy controls (light gray bar, n = 6) and CCR5Δ32 individuals (dark gray bar, n = 6). Data are expressed as number of cells (x106).
(B) Surface expression of CD45, CD11c and CD14 in primary monocytes (pMono, white bar, n = 6) and iMono generated during the differentiation of iPSCs of controls (iMono-Con, light gray bar, n = 6) and CCR5Δ32 individuals (iMono-CCR5Δ32, dark gray bar, n = 6). Data are expressed as frequency of positive cells (upper row) and Mean Fluorescence Intensity (MFI) (lower row). Data are presented as mean ± SD. Statistical significance was calculated using ANOVA.