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. 2023 Oct 24;26(12):108308. doi: 10.1016/j.isci.2023.108308
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit Anti-Cdk4 (C-22) Polyclonal Antibody Santa Cruz Biotechnology Cat# sc-260; RRID:AB_631219
Anti-β-Actin Antibody (C4) Santa Cruz Biotechnology Cat# sc-47778; RRID:AB_626632
Anti-Hsp70 (BB70 from Dr. David Toft, Mayo Clinic, Rochester, MN) N/A N/A
Anti-Hsp90β (H90.10 from Dr. David Toft, Mayo Clinic, Rochester, MN) N/A N/A
Anti-FKBP52 (His52C from Dr. David F. Smith, Mayo Clinic, Scottsdale, AZ) N/A N/A
Anti-P23 (JJ3 from David Toft) N/A N/A
Anti-HOP (F5 from David Smith) N/A N/A
Akt (pan) (C67E7) Rabbit mAb Cell Signaling Technology Cat# 4691; RRID:AB_915783
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb Cell Signaling Technology Cat# 4060; RRID:AB_2315049
LC3B (D11) XP Rabbit mAb Cell Signaling Technology Cat# 3868; RRID:AB_2137707
Autophagy Cleaved-LC3 Antibody (APG8a) Abcepta Cat# AP1805a; RRID:AB_2137587
Rabbit Anti-HSP70 Polyclonal Antibody, Unconjugated Bioss Cat# bs-0126R; RRID:AB_10852592
HSP27 antibody Proteintech Cat# 18284-1-AP; RRID:AB_2295540
GAPDH (D-6) Santa Cruz Biotechnology Cat# sc-166545; RRID:AB_2107299
ErbB-3 (C-17) (Anti-HER3) Santa Cruz Biotechnology Cat# sc-285, RRID:AB_2099723
Calreticulin Polyclonal Antibody Thermo Fisher Scientific Cat# PA3-900; RRID:AB_325990
Human/Mouse CX3CR1 Affinity Purified Polyclonal Ab R and D Systems Cat# AF5825; RRID:AB_2292441
Brilliant Violet 421(TM) anti-mouse CX3CR1 BioLegend Cat# 149023; RRID:AB_2565706
Purified anti-mouse CD45 BioLegend Cat# 103102; RRID:AB_312967
Brilliant Violet 605(TM) anti-mouse CD45 BioLegend Cat# 103139; RRID:AB_2562341
ILK BD Biosciences Cat# 611803; RRID:AB_399283
Purified anti-mouse CD8b.2 BioLegend Cat# 140402; RRID:AB_10645339
Purified anti-mouse CD8a BioLegend Cat# 100801; RRID:AB_312762
Alexa Fluor(R) 700 anti-mouse CD8a BioLegend Cat# 100729; RRID:AB_493702
InVivoPlus anti-mouse PD-1 (CD279) Bio X Cell Cat# BE0146, RRID:AB_10949053
APC/Cyanine7 anti-mouse CD279 (PD-1) BioLegend Cat# 135223, RRID:AB_2563522
Purified anti-mouse FOXP3 BioLegend Cat# 126401, RRID:AB_1089120
HMG-1 (HAP46.5) (HMGB-1) Santa Cruz Biotechnology Cat# sc-56698, RRID:AB_783817

Chemicals, peptides, and recombinant proteins

17-allylamino-demethoxygeldanamycin (17-AAG) ChemieTek Cat# #75747-14-7
5-[3-[4-(4-Chlorophenyl)-1-piperidinyl]propyl]pyrrolo[1,2-a]quinoxalin-4(5H)-one hydrochloride (JMS-17-2) Tocris Bioscience Cat# 6378/50
Cyclo[N-methyl-L-isoleucyl-N-oxa-D-valyl-N-methyl-L-isoleucyl-N-oxa-D-valyl-N-methyl-L-isoleucyl-N-oxa-D-valyl] (Enniatin A) Sigma Cat #E9661
Recombinant Mouse IFN-gamma Protein R&D Systems Cat #: 485-MI

Critical commercial assays

CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#: G3580
MagniSort Mouse T cell Enrichment kit Invitrogen Cat#: 8804-6820
RNA to cDNA EcoDry™ Premix (Double Primed) Takara Cat#: 639549
PureLink RNA Mini Kit ThermoFisher Cat#: 12183018A
PureLink DNase Set ThermoFisher Cat#: 12185010
TruSeq Stranded mRNA Library prep kit Illumina Cat#:20020594

Experimental models: Cell lines

Hs 578T ATCC RRID:CVCL_0332
EO771 ATCC Cat# CRL-3461, RRID:CVCL_GR23
4T1 ATCC CRL-2539, RRID:CVCL_0125)
MDA-MB-231 ATCC RRID:CVCL_0062
MDA-MB-453 ATCC RRID:CVCL_0418
MCF-7 ATCC RRID:CVCL_0031

Experimental models: Organisms/strains

BALB/cJ The Jackson Laboratory Cat# JAX:000651, RRID:IMSR_JAX:000651
C57BL/6J The Jackson Laboratory Cat# JAX:000664, RRID:IMSR_JAX:000664
NU/J The Jackson Laboratory Cat# JAX:002019, RRID:IMSR_JAX:002019

Software and algorithms

ImageJ https://imagej.net/ RRID:SCR_003070
GraphPad Prism http://www.graphpad.com/ RRID:SCR_002798
BIAevaluation Software https://www.biacore.com/lifesciences/service/downloads/software_licenses/biaevaluation/ RRID:SCR_015936
FlowJo software https://www.flowjo.com/solutions/flowjo RRID:SCR_008520

Oligonucleotides

mCx3cr1.F GAGTATGACGATTCTGCTGAGG Eurofins N/A
mCx3cr1.R CAGACCGAACGTGAAGACGAG Eurofins N/A
mCD74.F AGTGCGACGAGAACGGTAAC Eurofins N/A
mCD74.R CGTTGGGGAACACACACCA Eurofins N/A
mElmo1.F GAGAACAGCAGCCGAGAAGAT Eurofins N/A
mElmo1.R GTTGCAGGTCTCACTAGGCAG Eurofins N/A
mIl23a.F ATGCTGGATTGCAGAGCAGTA Eurofins N/A
mIl23a.R ACGGGGCACATTATTTTTAGTCT Eurofins N/A
mCxcr7.F AGCCTGGCAACTACTCTGACA Eurofins N/A
mCxcr7.R GAAGCACGTTCTTGTTAGGCA Eurofins N/A
mIL19. F CTCCTGGGCATGACGTTGATT Eurofins N/A
mIL19.R GCATGGCTCTCTTGATCTCGT Eurofins N/A
mCcl26.F TTCTTCGATTTGGGTCTCCTTG Eurofins N/A
mCcl26.R GTGCAGCTCTTGTCGGTGAA Eurofins N/A
mMtrf1l.F GGGACACGGAGTCATTGCTG Eurofins N/A
mMtrf1l.R TCTTCCGAAGGAACCAACAGT Eurofins N/A
mCCL4.F TTCCTGCTGTTTCTCTTACACCT Eurofins N/A
mCCL4.R CTGTCTGCCTCTTTTGGTCAG Eurofins N/A
mCxcl10.F CCAAGTGCTGCCGTCATTTTC Eurofins N/A
mCxcl10.R GGCTCGCAGGGATGATTTCAA Eurofins N/A
mCCL5.F GCTGCTTTGCCTACCTCTCC Eurofins N/A
mCCL5.R TCGAGTGACAAACACGACTGC Eurofins N/A
mCCL25.F TTACCAGCACAGGATCAAATGG Eurofins N/A
mCCL25.R CGGAAGTAGAATCTCACAGCAC Eurofins N/A
mCXCL1.F CTGGGATTCACCTCAAGAACATC Eurofins N/A
mCXCL1.R CAGGGTCAAGGCAAGCCTC Eurofins N/A
mTNF.R CCCTCACACTCAGATCATCTTCT Eurofins N/A
mTNF.R GCTACGACGTGGGCTACAG Eurofins N/A
mIL6.F TAGTCCTTCCTACCCCAATTTCC Eurofins N/A
mIL6.R TTGGTCCTTAGCCACTCCTTC Eurofins N/A
mIL12b.F TGGTTTGCCATCGTTTTGCTG Eurofins N/A
mIL12b.R ACAGGTGAGGTTCACTGTTTCT Eurofins N/A
mIL1B.F GCAACTGTTCCTGAACTCAACT Eurofins N/A
mIL1B.R ATCTTTTGGGGTCCGTCAACT Eurofins N/A
mIL4.F GGTCTCAACCCCCAGCTAGT Eurofins N/A
mIL4.R GCCGATGATCTCTCTCAAGTGAT Eurofins N/A
mIL10.F GCTCTTACTGACTGGCATGAG Eurofins N/A
mIL10.R CGCAGCTCTAGGAGCATGTG Eurofins N/A