Antibodies |
|
Rabbit Anti-Cdk4 (C-22) Polyclonal Antibody |
Santa Cruz Biotechnology |
Cat# sc-260; RRID:AB_631219
|
Anti-β-Actin Antibody (C4) |
Santa Cruz Biotechnology |
Cat# sc-47778; RRID:AB_626632
|
Anti-Hsp70 (BB70 from Dr. David Toft, Mayo Clinic, Rochester, MN) |
N/A |
N/A |
Anti-Hsp90β (H90.10 from Dr. David Toft, Mayo Clinic, Rochester, MN) |
N/A |
N/A |
Anti-FKBP52 (His52C from Dr. David F. Smith, Mayo Clinic, Scottsdale, AZ) |
N/A |
N/A |
Anti-P23 (JJ3 from David Toft) |
N/A |
N/A |
Anti-HOP (F5 from David Smith) |
N/A |
N/A |
Akt (pan) (C67E7) Rabbit mAb |
Cell Signaling Technology |
Cat# 4691; RRID:AB_915783
|
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb |
Cell Signaling Technology |
Cat# 4060; RRID:AB_2315049
|
LC3B (D11) XP Rabbit mAb |
Cell Signaling Technology |
Cat# 3868; RRID:AB_2137707
|
Autophagy Cleaved-LC3 Antibody (APG8a) |
Abcepta |
Cat# AP1805a; RRID:AB_2137587
|
Rabbit Anti-HSP70 Polyclonal Antibody, Unconjugated |
Bioss |
Cat# bs-0126R; RRID:AB_10852592
|
HSP27 antibody |
Proteintech |
Cat# 18284-1-AP; RRID:AB_2295540
|
GAPDH (D-6) |
Santa Cruz Biotechnology |
Cat# sc-166545; RRID:AB_2107299
|
ErbB-3 (C-17) (Anti-HER3) |
Santa Cruz Biotechnology |
Cat# sc-285, RRID:AB_2099723
|
Calreticulin Polyclonal Antibody |
Thermo Fisher Scientific |
Cat# PA3-900; RRID:AB_325990
|
Human/Mouse CX3CR1 Affinity Purified Polyclonal Ab |
R and D Systems |
Cat# AF5825; RRID:AB_2292441
|
Brilliant Violet 421(TM) anti-mouse CX3CR1 |
BioLegend |
Cat# 149023; RRID:AB_2565706
|
Purified anti-mouse CD45 |
BioLegend |
Cat# 103102; RRID:AB_312967
|
Brilliant Violet 605(TM) anti-mouse CD45 |
BioLegend |
Cat# 103139; RRID:AB_2562341
|
ILK |
BD Biosciences |
Cat# 611803; RRID:AB_399283
|
Purified anti-mouse CD8b.2 |
BioLegend |
Cat# 140402; RRID:AB_10645339
|
Purified anti-mouse CD8a |
BioLegend |
Cat# 100801; RRID:AB_312762
|
Alexa Fluor(R) 700 anti-mouse CD8a |
BioLegend |
Cat# 100729; RRID:AB_493702
|
InVivoPlus anti-mouse PD-1 (CD279) |
Bio X Cell |
Cat# BE0146, RRID:AB_10949053
|
APC/Cyanine7 anti-mouse CD279 (PD-1) |
BioLegend |
Cat# 135223, RRID:AB_2563522
|
Purified anti-mouse FOXP3 |
BioLegend |
Cat# 126401, RRID:AB_1089120
|
HMG-1 (HAP46.5) (HMGB-1) |
Santa Cruz Biotechnology |
Cat# sc-56698, RRID:AB_783817
|
|
Chemicals, peptides, and recombinant proteins |
|
17-allylamino-demethoxygeldanamycin (17-AAG) |
ChemieTek |
Cat# #75747-14-7 |
5-[3-[4-(4-Chlorophenyl)-1-piperidinyl]propyl]pyrrolo[1,2-a]quinoxalin-4(5H)-one hydrochloride (JMS-17-2) |
Tocris Bioscience |
Cat# 6378/50 |
Cyclo[N-methyl-L-isoleucyl-N-oxa-D-valyl-N-methyl-L-isoleucyl-N-oxa-D-valyl-N-methyl-L-isoleucyl-N-oxa-D-valyl] (Enniatin A) |
Sigma |
Cat #E9661 |
Recombinant Mouse IFN-gamma Protein |
R&D Systems |
Cat #: 485-MI |
|
Critical commercial assays |
|
CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) |
Promega |
Cat#: G3580 |
MagniSort Mouse T cell Enrichment kit |
Invitrogen |
Cat#: 8804-6820 |
RNA to cDNA EcoDry™ Premix (Double Primed) |
Takara |
Cat#: 639549 |
PureLink RNA Mini Kit |
ThermoFisher |
Cat#: 12183018A |
PureLink DNase Set |
ThermoFisher |
Cat#: 12185010 |
TruSeq Stranded mRNA Library prep kit |
Illumina |
Cat#:20020594 |
|
Experimental models: Cell lines |
|
Hs 578T |
ATCC |
RRID:CVCL_0332 |
EO771 |
ATCC |
Cat# CRL-3461, RRID:CVCL_GR23 |
4T1 |
ATCC |
CRL-2539, RRID:CVCL_0125) |
MDA-MB-231 |
ATCC |
RRID:CVCL_0062 |
MDA-MB-453 |
ATCC |
RRID:CVCL_0418 |
MCF-7 |
ATCC |
RRID:CVCL_0031 |
|
Experimental models: Organisms/strains |
|
BALB/cJ |
The Jackson Laboratory |
Cat# JAX:000651, RRID:IMSR_JAX:000651 |
C57BL/6J |
The Jackson Laboratory |
Cat# JAX:000664, RRID:IMSR_JAX:000664 |
NU/J |
The Jackson Laboratory |
Cat# JAX:002019, RRID:IMSR_JAX:002019 |
|
Software and algorithms |
|
ImageJ |
https://imagej.net/ |
RRID:SCR_003070
|
GraphPad Prism |
http://www.graphpad.com/ |
RRID:SCR_002798
|
BIAevaluation Software |
https://www.biacore.com/lifesciences/service/downloads/software_licenses/biaevaluation/ |
RRID:SCR_015936
|
FlowJo software |
https://www.flowjo.com/solutions/flowjo |
RRID:SCR_008520
|
|
Oligonucleotides |
|
mCx3cr1.F GAGTATGACGATTCTGCTGAGG |
Eurofins |
N/A |
mCx3cr1.R CAGACCGAACGTGAAGACGAG |
Eurofins |
N/A |
mCD74.F AGTGCGACGAGAACGGTAAC |
Eurofins |
N/A |
mCD74.R CGTTGGGGAACACACACCA |
Eurofins |
N/A |
mElmo1.F GAGAACAGCAGCCGAGAAGAT |
Eurofins |
N/A |
mElmo1.R GTTGCAGGTCTCACTAGGCAG |
Eurofins |
N/A |
mIl23a.F ATGCTGGATTGCAGAGCAGTA |
Eurofins |
N/A |
mIl23a.R ACGGGGCACATTATTTTTAGTCT |
Eurofins |
N/A |
mCxcr7.F AGCCTGGCAACTACTCTGACA |
Eurofins |
N/A |
mCxcr7.R GAAGCACGTTCTTGTTAGGCA |
Eurofins |
N/A |
mIL19. F CTCCTGGGCATGACGTTGATT |
Eurofins |
N/A |
mIL19.R GCATGGCTCTCTTGATCTCGT |
Eurofins |
N/A |
mCcl26.F TTCTTCGATTTGGGTCTCCTTG |
Eurofins |
N/A |
mCcl26.R GTGCAGCTCTTGTCGGTGAA |
Eurofins |
N/A |
mMtrf1l.F GGGACACGGAGTCATTGCTG |
Eurofins |
N/A |
mMtrf1l.R TCTTCCGAAGGAACCAACAGT |
Eurofins |
N/A |
mCCL4.F TTCCTGCTGTTTCTCTTACACCT |
Eurofins |
N/A |
mCCL4.R CTGTCTGCCTCTTTTGGTCAG |
Eurofins |
N/A |
mCxcl10.F CCAAGTGCTGCCGTCATTTTC |
Eurofins |
N/A |
mCxcl10.R GGCTCGCAGGGATGATTTCAA |
Eurofins |
N/A |
mCCL5.F GCTGCTTTGCCTACCTCTCC |
Eurofins |
N/A |
mCCL5.R TCGAGTGACAAACACGACTGC |
Eurofins |
N/A |
mCCL25.F TTACCAGCACAGGATCAAATGG |
Eurofins |
N/A |
mCCL25.R CGGAAGTAGAATCTCACAGCAC |
Eurofins |
N/A |
mCXCL1.F CTGGGATTCACCTCAAGAACATC |
Eurofins |
N/A |
mCXCL1.R CAGGGTCAAGGCAAGCCTC |
Eurofins |
N/A |
mTNF.R CCCTCACACTCAGATCATCTTCT |
Eurofins |
N/A |
mTNF.R GCTACGACGTGGGCTACAG |
Eurofins |
N/A |
mIL6.F TAGTCCTTCCTACCCCAATTTCC |
Eurofins |
N/A |
mIL6.R TTGGTCCTTAGCCACTCCTTC |
Eurofins |
N/A |
mIL12b.F TGGTTTGCCATCGTTTTGCTG |
Eurofins |
N/A |
mIL12b.R ACAGGTGAGGTTCACTGTTTCT |
Eurofins |
N/A |
mIL1B.F GCAACTGTTCCTGAACTCAACT |
Eurofins |
N/A |
mIL1B.R ATCTTTTGGGGTCCGTCAACT |
Eurofins |
N/A |
mIL4.F GGTCTCAACCCCCAGCTAGT |
Eurofins |
N/A |
mIL4.R GCCGATGATCTCTCTCAAGTGAT |
Eurofins |
N/A |
mIL10.F GCTCTTACTGACTGGCATGAG |
Eurofins |
N/A |
mIL10.R CGCAGCTCTAGGAGCATGTG |
Eurofins |
N/A |