Figure 3.

Representative photomicrographs (hematoxylin and eosin (H&E) staining) of liver tissues from chicken supplemented with nanomethionine and subjected to heat stress for 28 d. Livers tissues from control (A) and nanomethionine (B) revealed normal histopathologic structure of hepatic tissues. Hepatic tissues from heat stressed chickens showed diffuse moderate to severe vacuolization of hepatocytes mostly of hydropic type (red arrows), thickening of portal triads with the infiltrated mononuclear inflammatory cells (black arrows) (C), vascular and sinusoidal congestion and hemorrhage (arrow) (D). Hepatic tissues from heat stressed chicken treated with nanomethionine from d 1 showed mild thickening of portal areas with the congested portal veins (arrow) (E). Chickens in group subjected to heat stress and treated with nanomethionine at the heat stress time only showed focal areas of lytic necrosis infiltrated with mononuclear inflammatory cells and extravasated red blood cells (arrow) (F). Bar = 50 µm.