Figure 4.

Representative photomicrographs (hematoxylin and eosin (H&E) staining) of intestine from chicken supplemented with nanomethionine and subjected to heat stress for 28 d. Tissues from control (A) and nanomethionine groups (B) revealed normal histomorphologic structure of intestinal villi, associated crypt, and tunica muscularis, in addition to properly arranged columnar epithelium and goblet cells. Tissues from heat stressed chickens showed necrosis of villus epithelium (red arrow) and desquamation within the lumen (black arrow). Intestinal tissues from heat stressed chicken treated with nanomethionine either from d 1 (D) or at the heat stress time only (E) showed marked improvement in the histologic structure of intestine. Bar = 200 µm.