Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Steady-state kinetic parameters for hhCF-4OT and fCF-4OT.
| Substrate | Enzyme | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
|
| ||||
| 2-HM | hhCF-4OT | 30 ± 1 | 80 ± 7 | 3.7 × 105 |
| fCF-4OT | 180 ± 10 | 290 ± 25 | 6.2 × 105 | |
|
| ||||
| 5-Me-2-HM | hhCF-4OT | 6 ± 1 | 100 ± 20 | 6.0 × 104 |
| fCF-4OT | 11 ± 0.5 | 110 ± 10 | 1.0 × 105 | |
|
| ||||
| PP | hhCF-4OT | 60 ± 10 | 390 ± 65 | 1.5 × 105 |
| fCF-4OT | 140 ± 20 | 300 ± 50 | 4.7 × 105 | |
|
| ||||
| HPD | hhCF-4OT | 30 ± 3 | 620 ± 90 | 4.8 × 104 |
| fCF-4OT | 40 ± 5 | 330 ± 50 | 1.2 × 105 | |
|
| ||||
| CHM | - | - | - | - |
| fCF-4OT | 0.03 ± 0.004 | 250 ± 70 | 1.2 × 102 | |
