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Steady-state kinetic parameters for hhYR-4OT, fYR-4OT and hhYR-4OT variants using 2-HM.
| Enzyme | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|
|
| |||
| hhYR-4OT | 30 ± 10 | 170 ± 10 | 1.8 × 105 |
| fYR-4OT | 80 ± 5 | 260 ± 30 | 3.1 × 105 |
| αP1A | 0.4 ± 0.03 | 250 ± 30 | 1.6 × 103 |
| αR39A | 2.2 ± 0.1 | 300 ± 30 | 7.3 × 103 |
| βR11A | 0.030 ± 0.003 | 140 ± 20 | 2.1 × 102 |
| βR39A | 20 ± 1 | 260 ± 20 | 7.7 × 104 |
| βR62A | 1.3 ± 0.1 | 770 ± 100 | 1.7 × 103 |
