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Steady-state kinetic parameters for hhCF-4OT, fCF-4OT, and hhCF-4OT variants using 2-HM.
| Enzyme | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|
|
| |||
| hhCF-4OT | 30 ± 1 | 80 ± 10 | 3.8 × 105 |
| fCF-4OT | 180 ± 10 | 290 ± 25 | 6.2 × 105 |
| CFαP1A | 0.40 ± 0.04 | 120 ± 20 | 3.3 × 103 |
| CFαL11Y | 80 ± 4 | 190 ± 20 | 4.2 × 105 |
| CFαR39A | 15 ± 5 | 750 ± 210 | 2.0 × 104 |
| CFβR11A | 0.07 ± 0.004 | 140 ± 20 | 5.0 × 102 |
| CFβR39A | 40 ± 3 | 230 ± 25 | 1.7 × 105 |
| CFβR62A | 9 ± 1 | 400 ± 60 | 2.3 × 104 |
