Fig. 5.

Alterations of sncRNAs expression levels in RNase T2 KI sperm. A rsRNAs, tsRNAs, miRNAs, and piRNAs in sperm were detected by using small RNA-seq and compared between RNase T2 KI and control. B–E Volcano plots illustrated the differential expression of rsRNAs (B), tsRNAs (C), miRNAs (D), and piRNAs (E) in sperm between RNase T2 KI and control (n = 3). F The mRNA levels of RNase T2, Dicer, and ANG in caput epididymis indicated the highest expression of RNase T2 in the caput epididymis (n = 3). G The immunoblot results showed the abundant expression of RNase T2 in caput epididymis while its expression was significantly higher than two other ribonucleases: Dicer and ANG. Quantification of Western blots, normalized to β-actin (n = 3). Data are expressed as means ± SD; **P < 0.01