FIG. 2.

Nucleotide sequence of the purDEK region from L. lactis CHCC285. The nucleotide sequence of the 3,573-bp Sau3AI fragment present in pLN51. The translated amino acid sequences of four open reading frames are shown below the nucleotide sequence and are labelled by the gene designation. Putative ribosomal binding sites (SD) are shown in boldface type. The transcription start point is underlined and marked +1, and the presence of a putative −10 region is likewise indicated by underlining. The locations of various endpoints from exonuclease deletions are indicated by underlining of the last nucleotide present in the deletion plasmids and an arrow above the nucleotide sequence pointing toward the other end of the DNA fragment in the particular plasmid (indicated beside the arrow).