TABLE 1.
Strain and plasmid list
| Strain or plasmid | Genotype and/or relevant feature(s) | Source or reference |
|---|---|---|
| L. lactis | ||
| CHCC285 | Wild type | 21 |
| MG1363 | Plasmid free | 9 |
| MG1614 | MG1363 Rifr Strr | 9 |
| E. coli | ||
| DH5α | Φ80 lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 | 10 |
| LN3 | SØ609/λZAPII-purDEK | This study |
| SØ609 | ara thi rpsL Δ(pro-gpt-lac) hpt deoD purD | 13 |
| Plasmids | ||
| pBluescript KS(+/−) | Cloning vector Apr | Stratagene |
| pIC19H | Cloning vector Apr | ATCC 37408b |
| pAK80 | Eryr; promoterless lacLM | 11 |
| pLN48 | 7.2-kb KpnI fragment from LN3 inserted into pBluescript KS(−) | This study |
| pLN51 | 3.2-kb KpnI-SpeI fragment from pLN48 inserted into pBluescript KS(+) | This study |
| pLN67 | 0.9-kb EcoRI fragment from pLN51 inserted into pBluescript KS(+) | This study |
| pLN71 | 0.9-kb HindIII fragment from pLN67 (purD′) fused to lacLM in pAK80 | This study |
| pLN72 | 0.9-kb HindIII fragment from pLN67 (orf1′) fused to lacLM in pAK80 | This study |
| p10a | Exonuclease III deletion derivative of pLN71 | This study |
| pLN82 | 0.9-kb EcoRI fragment from pLN67 inserted in pIC19H | This study |
| pLN84 to pLN90 | Exonuclease III deletion derivatives of pLN82 | This study |
| pLN93 to pLN99 | HindIII-BamHI fragments from pLN84-90 fused to lacLM in pAK80 | This study |
a
p19, p22, p27, and p30 are also exonuclease III deletion derivatives of pLN71 (this study).
b
American Type Culture Collection, Rockville, Md.