FIG. 3.

Effects of temperature on trehalose phosphorolysis and synthesis of TSase. Error bars are included for the points for which the standard deviation was greater than 10% of the point’s value. (A) For determination of the optimum temperatures, the trehalose phosphorolysis (○) and synthesis (•) activities at the indicated temperatures were determined. The TSase activity for phosphorolysis at the optimal temperature was 0.011 U and TSase synthesized trehalose at 5.03 μmol/h at the optimal temperature. (B) For determination of heat resistance, the remaining phosphorolysis (○) and synthesis (•) activities were measured after the enzyme had been placed at the indicated temperatures for 30 min. The remaining synthesis activity was also measured at 35°C under the standard reaction conditions after the same treatment. A value of 0.01 U is equivalent to 100% activity for phosphorolysis, and a value of 2.99 μmol of trehalose per h is equivalent to 100% activity for synthesis.