FIG. 6.

Conversion of sucrose into trehalose by CES I. The reaction conditions were as follows: 300 mM each sucrose and glucose, 20 mM P_i, and 1.05 U of SPase and TSase each per ml in 100 mM MES buffer (pH 6.5); temperature, 37.5°C. The standard deviation for all the points was within 10% of the point’s value.