FIG. 7.
Conversion of sucrose into trehalose by CES II. The reaction mixture, which contained 300 mM sucrose, 20 mM MgSO4, 1.05 U of SPase, TSase, and GIase each per ml, and 20 mM Pi in 100 mM HEPES buffer (pH 7.0), was incubated at 30°C for the indicated periods, and the amounts of trehalose (○), sucrose (•), glucose (▵), and fructose (▴) were measured by HPLC. α-d-Glucose 1-P (□) was measured by the enzymatic method described in Materials and Methods. The standard deviation for all the points was within 10% of the point’s value.