TABLE 2.

Purification of trehalose synthase from G. frondosa

Step	Protein (mg)	Total activity (U)	Sp act (U)	Yield (%)	Purifi-cation (fold)
Crude extract	8,100	320	0.04	100	1
(NH4)2SO4 (0–40%)	6,400	270	0.04	85	1
Butyl-Toyopearl	1,100	200	0.19	63	5
SuperQ-Toyopearl	190	200	1.0	61	26
AF-Blue-Toyopearl	68	170	2.5	53	62
AF-Red-Toyopearl	49	160	3.2	49	80
SuperQ-Toyopearl	31	140	4.4	43	111
HPLC	27	110	4.2	36	106