Skip to main content
. 2023 Nov 10;19(11):e1011764. doi: 10.1371/journal.ppat.1011764

Fig 7. HEX mitigates SARA-induced inflammatory response and blood-milk damage in the mammary glands.

Fig 7

PBS, HEX, and rumen fluid from healthy cows, and SARA-associated mastitis cows were administered via gavage to mice for 10 days. Finally, the mammary gland tissues were collected for detection. A-B. The pathological changes and inflammatory scores in different groups are shown. C-E. MPO activity (C), and the levels of TNF-α (D) and IL-1β (E) were determined (n = 5). F-I. ZO-1, Occludin, and Claudin-3 protein levels and intensity analysis were performed (n = 5). J. Representative immunohistochemical image of the mammary glands by ZO-1, Occludin, and Claudin-3 antibodies staining (scale bars, 20 μm). One-way ANOVA (B-E and G-I) was performed and values are presented as mean ± SD (B-E and G-I). *p < 0.05, **p < 0.01, and ***p < 0.001 indicate marked difference from each group. ns, no significance.