FIG. 1.

Degradation of TCE by AEK301/pYK3021 at an initial concentration of 16 μM TCE (▴) and AEK301/pYK3021 at an initial concentration of 80 μM TCE (■). The negative control, AEK301 alone (○), was used at an initial concentration of 80 μM TCE. Cultures were grown in MMO supplemented with 10 mM sodium citrate to mid-log phase, harvested by centrifugation, and suspended in fresh medium to an optical density of 1.0 at 425 nm. After 1 h at 30°C, 2-ml samples of each strain were then distributed into vials and the vials were sealed. Reactions were initiated by the injection of TCE through the septum. Samples were collected in duplicate every 15 min for a total of 3 h. Each data point represents the average of two or more samples, and error bars are provided where visible.