TABLE 1.

Effects of carbon sources on growth of and TCE degradation by AEK301/pYK3021

Medium and carbon source	Concn of carbon source	Concn of TCE remaining (μM)a	% of TCE removed	Doubling time (h/generation)
				Without TCE	With 40 μM TCEc
MMO
Benzoate	2.5 mM	23.4	41.5	2.0	2.1
Casamino Acids	0.30%	12.3	69.3	1.8	1.8
Citrate	20 mM	1.2	97.0	2.6	2.5
Gluconate	20 mM	6.5	83.8	2.4	2.5
Lactate	20 mM	23.0	42.5	1.7	1.8
Malate	20 mM	37.4	6.5	1.6	1.7
Sodium citrate	20 mM	NDb	>99.9	2.5	2.4
TNB		38.5	3.8	1.2	1.2

^aAfter 2 h of incubation at 30°C; initial concentration, 40 μM TCE. 

^bND, not detected. 

^cTCE was added by injection into sealed cultures during early exponential phase.