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. 2023 Nov 15;623(7988):772–781. doi: 10.1038/s41586-023-06548-w

Fig. 5. Apparent stalling and redirection of mesenchyme differentiation in the Sox9 regulatory INV mutant.

Fig. 5

a, LochNESS distributions for all G4 mutants in the mesenchymal trajectory (left) and the Sox9 regulatory INV mutant in mesenchymal sub-trajectories (right). b, Model of the Sox9 regulatory INV mutation depicting ectopic Kcnj2 regulation through enhancer adoption. c, RNA velocity of mesenchymal G4 wild-type and Sox9 regulatory INV cells coloured by sub-trajectories (top) or genotype (bottom). d, Sox9 regulatory INV heterozygous mutant and littermate wild-type RNAscope images (red: Kcnj2; green: Sox9), with insets below highlighting a region corresponding to developing bone (area outlined with white dots line). Scale bars, 500 μm. e, RNA velocity of G4 wild-type and Sox9 regulatory INV cells in the limb mesenchymal sub-trajectory labelled by annotation (top) or genotype (bottom). f, The same as in e, but coloured by branch number. g, Dot plot of the top differentially expressed genes in the four branches shown in f.